• Product NameRecombinant Equine Interleukin-1 beta
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to a specific activity of > 5.0 x10⁷ IU/mg.
• EndotoxinLess than 1 EU/ug of rEqIL-1β as determined by LAL method.
• Alternative Names    IL-1 beta
• UniprotQ28386
• Gene ID100034237
• Calculated MW Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
• Target Sequence AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, 0.1 % Tween-80.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant equine interleukin-1 beta is a 17 kDa protein containing 153 amino acid residues and it shares 63 %~70 % a.a. sequence identity with canine, cotton rat, porcine, feline, human, mouse, rat, and rhesus IL1β.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting