• Product NameRecombinant Rat gamma-Interferon Inducible Protein 10/CXCL10
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.
• EndotoxinLess than 1 EU/ugof rRtIP-10/CXCL10 as determined by LAL method.
• Alternative Names    Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1
• UniprotP48973
• Gene ID245920
• Calculated MW Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
• Target Sequence IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in rat it is also named the Mob-1 gene. This gene was identified on the basis of differential expression in rat embryo fibroblasts. CXCL10 elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Rat CXCL10 shares approximately 68 % amino acid sequence identity with human CXCL10.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting