• Product NameRecombinant Human PCT/Procalcitonin
• Purification> 98 % by SDS-PAGE and HPLC analyses.
• Biological ActivityData is not available.
• EndotoxinLess than 0.1 EU/ugof rHuPCT/Procalcitonin as determined by LAL method.
• Calculated MW Approximately 12.8 kDa, a single non-glycosylated polypeptide chain containing 116 amino acids.
• Target Sequence APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- Refer to lot specific COA for the Use by Date when stored at ≤-20 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Procalcitonin (PCT) belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). PCT is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. PCT is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. But its level is related to the severity of bacterial sepsis, it is considered to be one of the earliest and most specific markers of sepsis.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting