• Product NameSIRT1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman
• SpecificitySIRT1 Antibody detects endogenous levels of total SIRT1
• Immunogen DescriptionA synthesized peptide derived from human SIRT1
• Alternative Names    hSIR2; hSIRT1; NAD-dependent deacetylase sirtuin 1; SIR1; SIR2-like protein 1; SIR2L1; sirtuin 1
• Accession No. Uniprot:Q96EB6
• UniprotQ96EB6
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30

• This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.

• Western blot analysis of Sir2/SIRT1 expression in Jurkat cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using SIRT1 Antibody.
• Immunofluorescent analysis of Hela cells, using SIRT1 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting