• Product NameIKB alpha Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityIKB alpha Antibody detects endogenous levels of total IKB alpha
• Immunogen DescriptionA synthesized peptide derived from human IKB alpha
• Alternative Names    I-kappa-B-alpha; NFKBI; NFKBIA; NF-kappaB inhibitor alpha; IKBA; IkappaBalpha; MAD3; RL/IF-1;
• Accession No. Uniprot:P25963
• UniprotP25963
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30

• NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm.

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm.

• Western blot analysis of IKB alpha expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse stomach, using IKB alpha Antibody.
• Immunofluorescent analysis of Hela cells, using IKB alpha Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting