• Product NameCleaved PARP Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificityThis antibody is specific for the p25 cleaved form of human PARP.
• Immunogen DescriptionA synthesized peptide derived from human Cleaved PARP
• Alternative Names    PPOL; ADPRT; ADPRT1; pADPRT-1; PARP; PARP-1;
• Accession No. Uniprot:P09874
• UniprotP09874
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:100

• Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

• Western blot analysis of Cleaved PARP expression in (1) RAW264.7 cell lysate;(2) NIH/3T3 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using Cleaved PARP Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting