• Product NameCaspase-9 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC IP
• Species ReactivityHuman
• SpecificityCaspase-9 Antibody detects endogenous levels of total Caspase-9
• Immunogen DescriptionA synthesized peptide derived from human Caspase-9
• Alternative Names    APAF-3; Caspase9; ICE-LAP6; Apoptotic protease Mch-6; CASP-9; Caspase-9; Caspase-9 precursor; MCH6; RNCASP9;
• Accession No. Uniprot:P55211
• UniprotP55211
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

• Western blot analysis of Caspase-9 in HeLa cell lysate treated with Camptothecin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody.
• Immunofluorescent analysis of 3T3 cells, using Caspase-9 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting