• Product NameChk1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityChk1 Antibody detects endogenous levels of total Chk1
• Immunogen DescriptionA synthesized peptide derived from human Chk1
• Alternative Names    Serine/threonine-protein kinase Chk1; CHEK1; CHK1
• Accession No. Uniprot:O14757
• UniprotO14757
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:30

• DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

• Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting