• Product NameActin (Alpha-Actin) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityActin (Alpha-Actin) Antibody detects endogenous levels of total Actin (Alpha-Actin)
• Immunogen DescriptionA synthesized peptide derived from human Actin (Alpha-Actin)
• Alternative Names    ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1
• Accession No. Uniprot:P68133
• UniprotP68133
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

• Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody.
• Immunofluorescent analysis of Hela cells, using Actin (Alpha-Actin) Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting