• Product NameNotch1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse
• SpecificityNotch1 Antibody detects endogenous levels of total Notch1
• Immunogen DescriptionA synthesized peptide derived from human Notch1
• Alternative Names    NOTC1, NOTCH1, TAN1, hN1, neurogenic locus notch homolog protein 1, neurogenic locus notch homolog protein 1 precursor,
• Accession No. Uniprot:P46531
• UniprotP46531
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:3000 IHC 1:50~1:200 ICC/IF 1:100~1:500 FC 1:200~1:500

• Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

• Western blot analysis of NOTCH1 expression in HEK293 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .
• Immunofluorescent analysis of HeLa cells, using Notch1 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting