• Product NameHistone H2A.X Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityHistone H2A.X Antibody detects endogenous levels of total Histone H2A.X
• Immunogen DescriptionA synthesized peptide derived from human Histone H2A.X
• Alternative Names    H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; AW228881; H2A histone family member X; Histone 2A;
• Accession No. Uniprot:P16104
• UniprotP16104
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of Histone H2A.X expression in Raji cell lysates.
• Immunohistochemical analysis of paraffin-embedded rat lung, using Histone H2A.X Antibody.
• Immunofluorescent analysis of Hela cells, using Histone H2A.X Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting