• Product NamePaxillin Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityPaxillin Antibody detects endogenous levels of total Paxillin
• Immunogen DescriptionA synthesized peptide derived from human Paxillin
• Alternative Names    Paired box protein Pax-1; PAX-1; PAXI; paxillin; paxillin alpha;
• Accession No. Uniprot:P49023
• UniprotP49023
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:100 ICC/IF 1:50~1:100 IP 1:50

• Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). PXN is a multi-domain cytoskeletal protein that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions.

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). PXN is a multi-domain cytoskeletal protein that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions.

• Western blot analysis of Paxillin expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Paxillin Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting