• Product NameAlkaline Phosphatase Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityAlkaline Phosphatase Antibody detects endogenous levels of total Alkaline Phosphatase
• Immunogen DescriptionA synthesized peptide derived from human Alkaline Phosphatase
• Alternative Names    Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;
• Accession No. Uniprot:P05186
• UniprotP05186
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50 FC 1:50

• There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

• Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate.
• Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
• Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting