• Product NameHsp70 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityHsp70 Antibody detects endogenous levels of total Hsp70
• Immunogen DescriptionA synthesized peptide derived from human Hsp70
• Alternative Names    HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HSPA1A;
• Accession No. Uniprot:P0DMV8
• UniprotP0DMV8
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:100~1:500 ICC/IF 1:50~1:100 FC 1:100

• In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

• Western blot analysis of HSP70 expression in (1) K562 cell lysate; (2) 293T cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody .
• Immunofluorescent analysis of Hela cells, using Hsp70 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting