• Product NameNestin Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsIHC ICC/IF
• Species ReactivityHuman
• SpecificityNestin Antibody detects endogenous levels of Nestin
• Immunogen DescriptionA synthesized peptide derived from human Nestin
• Alternative Names    NES; Nestin; Nbla00170; ESTM 46; NEST;
• Accession No. Uniprot:P48681
• UniprotP48681
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• IHC 1:50~1:200 ICC/IF 1:50~1:200

• May help to regulate cell structure and intracellular processes required for neural cell division and migration. Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins. Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells.

May help to regulate cell structure and intracellular processes required for neural cell division and migration. Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins. Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells.

• Immunohistochemical analysis of paraffin-embedded human kidney, using Nestin Antibody.
• Immunofluorescent analysis of PC12 cells, using Nestin Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting