• Product Namepro Caspase 3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse
• Specificitypro Caspase 3 Antibody detects endogenous levels of pro Caspase 3
• Immunogen DescriptionA synthesized peptide derived from human pro Caspase 3
• Alternative Names    Yama; CASP3; Caspase 3; CPP32; Caspase 3 apoptosis related cysteine peptidase; CPP32B; Procaspase 3; SCA 1; SCA1; Yama protein;
• Accession No. Uniprot:P42574
• UniprotP42574
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• Caspases are a family of cytosolic aspartate specific cysteine proteases. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.

Caspases are a family of cytosolic aspartate specific cysteine proteases. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.

• Western blot analysis of pro Caspase 3 expression in Jurkat cell lysate.
• Immunohistochemical analysis of paraffin-embedded human colon, using pro Caspase 3 Antibody.
• Immunofluorescent analysis of Hela cells, using pro Caspase 3 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting