• Product NameATG5 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityATG5 Antibody detects endogenous levels of ATG5
• Immunogen DescriptionA synthesized peptide derived from human ATG5
• Alternative Names    APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; ASP; ATG 5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5;
• Accession No. Uniprot:Q9H1Y0
• UniprotQ9H1Y0
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.

Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.

• Western blot analysis of ATG5 expression in Raji cell lysate.
• Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ATG5 Antibody.
• Immunofluorescent analysis of Raji cells, using ATG5 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting