• Product NameEstrogen Receptor beta Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.22B11
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman
• SpecificityEstrogen Receptor beta Antibody detects endogenous levels of total Estrogen Receptor beta
• Immunogen DescriptionA synthesized peptide derived from human Estrogen Receptor beta
• Target NameESR2
• Alternative Names    ESR2; ESTRB; Estrogen receptor beta; NR3A2;
• Accession No. Uniprot:Q92731
• UniprotQ92731
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200

• Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).

• All Lanes:Estrogen Receptor beta Antibody at 1/ 2000 dilution.Lane1:293.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 59kDaObserved band size: 59kDa
• All Lanes:Estrogen Receptor beta Antibody at 1/ 2000 dilution.Lane1:SH-SY5Y.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 59kDaObserved band size: 59kDa
• All Lanes:Estrogen Receptor beta Antibody at 1/ 2000 dilution.Lane1:MCF-7 Cell Lysate.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 59kDaObserved band size: 59kDa
• Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Estrogen Receptor beta Antibody.Tris-EDTA Buffer (pH9.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting