• Product NameAtg7(Apg7) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityAtg7(Apg7) Antibody detects endogenous levels of total Atg7(Apg7)
• Immunogen DescriptionA synthesized peptide derived from human Atg7(Apg7)
• Alternative Names    hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L;
• Accession No. Uniprot:O95352
• UniprotO95352
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 ICC/IF 1:50~1:200

• Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

• Western blot analysis of Atg7(Apg7) expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting