• Product NameMDM2(phospho-Ser166) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2.
• Target NameMDM2
• ModificationPhospho
• Alternative Names    HDMX, hdm2
• Accession No. Swiss-Prot: Q00987
  NCBI Protein: NP_002383.2
• UniprotQ00987
• Gene ID4193;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 90kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.

• Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2(phospho-Ser166) Antibody #11550.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Phospho-Ser166) Antibody #11550(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using MDM2(phospho-Ser166) Antibody #11550.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting