• Product NameCD33 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms Rt
• SpecificityAt least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 14 amino acid peptide near the center of human CD33.
• Target NameCD33
• Alternative Names    Myeloid surface antigen CD33, p67, SIGLEC3
• Accession No. Swiss-Prot:P20138
  Gene ID:945
• UniprotP20138
• Gene ID945;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer’s disease.

• Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting