• Product NameEstrogen Receptor-a Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB;IHC;IF
• Species ReactivityHu Ms Rt
• SpecificityERα Polyclonal Antibody detects endogenous levels of ERα protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha.
• Target NameEstrogen Receptor-a
• Alternative Names    ER; ESR; ESR1; ESTR; ESTRA
• Accession No. Swiss-Prot: P03372
  NCBI Protein: NP_000116.2
• UniprotP03372
• Gene ID2099;
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. 

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.

• Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a(Ab-106) Antibody #21066 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-106) Antibody #21066(left) or the same antibody preincubated with blocking peptide(right).
• Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
• Immunofluorescence analysis of Hela cell.
• Immunofluorescence analysis of rat-lung tissue.
• Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
• Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting