• Product NameCX3CR1 Antibody FITC Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsFlow-Cyt IF
• Species ReactivityMs Rt
• Immunogen DescriptionKLH conjugated synthetic peptide derived from human CX3CR1
• ConjugatesFITC
• Excitation Emission494nm 518nm
• Target NameCX3CR1
• Alternative Names    V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1; CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13
• Accession No. Swiss-Prot#P49238
  NCBI Gene ID1524
• UniprotP49238
• Gene ID1524;
• Cell Localization Cell membrane; Multi-pass membrane protein.
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• Flow-Cyt=2ug/Test

Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

• Blank control:U937.Primary Antibody (green line): Rabbit Anti-CX3CR1 antibodyDilution: 2μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
• Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
• Blank control:U251.Primary Antibody (green line): Rabbit Anti-CX3CR1 antibodyDilution: 2μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting