• Product NameAGR2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total AGR2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameAGR2
• Alternative Names    Anterior gradient protein 2 homolog, AG2, GOB-4, HAG-2, XAG-2, PDIA17
• Accession No. Swiss-Prot:O95994
  Gene ID:10551;
• UniprotO95994
• Gene ID10551;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 20kd
  ELISA: 1:1000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:10-1:50

Anterior gradient protein 2 homolog (AGR2) is a human protein originally discovered in Xenopus laevis, encoded for by the gene of the same name.In Xenopus AGR2 is an important role in cement gland differentiation, however in human cancer cell lines high levels of AGR2 correlate with reduction of the p53 response and AGR2 levels are elevated in the preneoplastic tissue Barrett's oesophagus.

• Gel: 10%SDS-PAGE
  Lysate: 40 µg Mouse Large intestine tissue lysate
  Primary antibody: 1/1000 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
  Exposure time: 20 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using 31005 (AGR2 Antibody) at dilution 1/10, on the right is treated with the fusion protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting