• Product NameCAP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total CAP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 319-453 amino acids of Human Adenylyl cyclase-associated protein 1
• Target NameCAP1
• Alternative Names    Adenylyl cyclase-associated protein 1, CAP, CAP1-PEN
• Accession No. Swiss-Prot:Q01518
  Gene ID:10487;
• UniprotQ01518
• Gene ID10487;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 52kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.

• Gel: 10%SDS-PAGE
  Lysate: 30 µg NIH/3T3 cell lysate
  Primary antibody: 1/150 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31042 (CAP1 Antibody) at dilution 1/25, on the right is treated with the fusion protein.
  
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting