• Product NameCA2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total CA2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameCA2
• Alternative Names    carbonic anhydrase II, CAII, Car2, CA-II
• Accession No. Swiss-Prot:P00918
  Gene ID:760;
• UniprotP00918
• Gene ID760;
• Concentration 0.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 29kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:25-1:100

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Essential for bone resorption and osteoclast differentiation By similarity. Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.

• Gel: 10%+12%SDS-PAGE
  Lysate: 40 µg Mouse brain tissue lysate
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 second
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31044 (CA2 Antibody) at dilution 1/20, on the right is treated with the fusion protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting