• Product NameCDC20 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total CDC20 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 182-480 amino acids of Human Cell division cycle protein 20 homolog
• Target NameCDC20
• Alternative Names    Cell division cycle protein 20 homolog, CDC20A, p55CDC, bA276H19.3
• Accession No. Swiss-Prot:Q12834
  Gene ID:991;
• UniprotQ12834
• Gene ID991;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 55kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:10-1:50

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.

• Gel: 10%SDS-PAGE
  Lane1: 293T cell lysate
  Lane2: Human cervical cancer tissue lysate
  Lysates: 30ug per lane
  Primary antibody: 1/600 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
  Exposure time: 30 seconds
  
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31052(CDC20 Antibody) at dilution 1/10, on the right is treated with the fusion protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting