• Product NameHPSE Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total HPSE protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a C terminal 250 amino acids of human heparanase
• Target NameHPSE
• Alternative Names    heparanase, HPA, HPA1, HPR1, HSE1, HPSE1
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 61kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

• Gel: 10%SDS-PAGE
  Lysate: 60 µg Human transitional cell carcinoma tissue lysate
  Primary antibody: 1/100 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 5 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting