• Product NameJAK1(Ab-1022) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total JAK1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 1020~1024 (K-E-Y-Y-T) derived from Human JAK1.
• Target NameJAK1
• Alternative Names    Janus kinase 1
• Accession No. Swiss-Prot: P23458
  NCBI Protein: NP_002218.2
• UniprotP23458
• Gene ID3716;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 130kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Wang R, et al. (2003) Arch Biochem Biophys. 410(1): 7-15.

• Western blot analysis of extracts from 3T3, HepG2, HT29 and MDA cells using JAK1(Ab-1022) Antibody #21119.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK1(Ab-1022) Antibody #21119(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting