• Product NameSPP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total SPP1 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 17-300 amino acids of human secreted phosphoprotein 1
• Target NameSPP1
• Alternative Names    secreted phosphoprotein 1, OPN, BNSP, BSPI, ETA-1
• Accession No. Swiss-Prot:P10451
  Gene ID:6696;
• UniprotP10451
• Gene ID6696;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 34kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

• Gel: 10%SDS-PAGE
  Lane1: 231 cell lysate
  Lane2: OP9 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting