• Product NamePSMD14 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total PSMD14 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 216-310 amino acids of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Target NamePSMD14
• Alternative Names    Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, PAD1; POH1; RPN11
• Accession No. Swiss-Prot:O00487
  Gene ID:10213;
• UniprotO00487
• Gene ID10213;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 35kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2.

• Gel: 10%SDS-PAGE
  Lane1: 293T cell lysate
  Lane2: SKOV3 cell lysate
  Lane3: NIH/3T3 cell lysate
  Lane4: 231 cell lysate
  Lysates: 50 ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using 31120(PSMD14 Antibody) at dilution 1/20, on the right is treated with the fusion protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting