• Product NamePKD/PKCm(Ab-738) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PKD/PKCm protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 736~740 (E-K-S-F-R) derived from Human PKD/PKCm.
• Target NamePKD/PKCm
• Alternative Names    nPKC-D1; Protein kinase D; Protein kinase C mu type; nPKC-mu;
• Accession No. Swiss-Prot: Q15139
  NCBI Protein: NP_002733.2
• UniprotQ15139
• Gene ID5587;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 115kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.

Storz P, et al. Mol Cell Biol. 2004 Apr; 24(7): 2614-2626.
Storz P, et al. Mol Cell Biol. 2005 Oct; 25(19): 8520-8530.
Zhang W, et al. J Biol Chem 2005 May 13; 280(19): 19036-19044

• Western blot analysis of extracts from Hela, 293, THP1 and JK cells using PKD/PKCm(Ab-738) Antibody #21126.
• Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Ab-738) Antibody #21126.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting