• Product NameAVEN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total AVEN protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 339-353 amino acids of Human Apoptosis, caspase activation inhibitor
• Target NameAVEN
• Alternative Names    Apoptosis, caspase activation inhibitor, PDCD12
• Accession No. Swiss-Prot:Q9NQS1
  Gene ID:57099;
• UniprotQ9NQS1
• Gene ID57099;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 39kd
  ELISA: 1:500-1:5000
  Western blotting: 1:500-1:1000

Aven binds to both Bcl-x(L) and the caspase regulator Apaf-1,which can supported that Bcl-x(L) inhibits cell death at a late (postmitochondrial) step in the death pathway.Identified in a yeast two-hybrid screen,Aven inhibited the proteolytic activation of caspases in a cell-free extract and suppressed apoptosis induced by Apaf-1 plus caspase-9. Expressing highly in the testis, ovary, and thymus .

• Gel: 10%SDS-PAGE
  Lysate: 40 µg Jurkat cell lysate
  Primary antibody: 1/400 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 50 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting