• Product NameCSNK2B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CSNK2B protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 201-215 amino acids of Human Casein kinase II subunit beta
• Target NameCSNK2B
• Alternative Names    Casein kinase II subunit beta, G5A, CK2B, CK2N, CSK2B
• Accession No. Swiss-Prot:P67870
  Gene ID:1460;
• UniprotP67870
• Gene ID1460;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 25kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:10-1:50

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.

• Gel: 10%SDS-PAGE
  Lysate: 30 µg Hela cell lysate
  Primary antibody: 1/1000 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31171 (CSNK2B Antibody) at dilution 1/25, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting