• Product NameDISC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total DISC1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 737-750 amino acids of Human disrupted in schizophrenia 1
• Target NameDISC1
• Alternative Names    disrupted in schizophrenia 1, SCZD9, C1orf136
• Accession No. Swiss-Prot:Q9NRI5
  Gene ID:27185;
• UniprotQ9NRI5
• Gene ID27185;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 60kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Gel: 8%SDS-PAGE
  Lysate: 40 µg 293T cell lysate
  Primary antibody: 1/1200 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31185 (DISC1 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting