• Product NameHDAC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total HDAC1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 469-482 amino acids of Human histone deacetylase 1
• Target NameHDAC1
• Alternative Names    histone deacetylase 1, HD1, RPD3, GON-10, RPD3L1
• Accession No. Swiss-Prot:Q13547
  Gene ID:3065;
• UniprotQ13547
• Gene ID3065;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 55kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

• Gel: 10%SDS-PAGE
  Lane1: Human colon tissue lysate
  Lane2: Human kidney cancer tissue lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 31215 (HDAC1 Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting