• Product NameHMGB3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total HMGB3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 164-178 amino acids of Human high mobility group box 3
• Target NameHMGB3
• Alternative Names    high mobility group box 3, HMG4, HMG-4, HMG2A, HMG-2a
• Accession No. Swiss-Prot:O15347
  Gene ID:3149;
• UniprotO15347
• Gene ID3149;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 23kd
  ELISA: 1:1000-1:10000
  Western blotting: 1:2000-1:5000
  Immunohistochemistry: 1:20-1:100

High-mobility group protein B3 is a protein that in humans is encoded by the HMGB3 gene. HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 and HMG2, HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription.

• Gel: 10%SDS-PAGE
  Lane1: 293T cell lysate
  Lane2: Hela cell lysate
  Lane3: Mouse lung tissue lysate
  Lane4: Mouse brain tissue lysate
  Lysates: 30ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1.5 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31220 (HMGB3 Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting