• Product NamePIF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PIF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 9-28 amino acids of Human ATP-dependent DNA helicase PIF1
• Target NamePIF1
• Alternative Names    ATP-dependent DNA helicase PIF1, PIF, C15orf20
• Accession No. Swiss-Prot:Q9H611
  Gene ID:80119;
• UniprotQ9H611
• Gene ID80119;
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 70kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000

PIF1 is a 5-prime-to-3-prime DNA helicase that negatively regulates telomerase (see TERT; MIM 187270), a reverse transcriptase that maintains telomere length. Tightly cell cycle regulated and expressed in late S/G2 phase.

• Gel: 8%SDS-PAGE
  Lane1: 293T cell lysate
  Lane2: Hela cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 1 minute
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting