• Product NameMYL9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total MYL9 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 2-14 amino acids of Human myosin, light chain 9, regulatory
• Target NameMYL9
• Alternative Names    myosin, light chain 9, regulatory, LC20, MLC2, MRLC1, MYRL2, MLC-2C
• Accession No. Swiss-Prot:P24844
  Gene ID:10398;
• UniprotP24844
• Gene ID10398;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 14kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

• Gel: 12%SDS-PAGE
  Lane1: Jurkat cell lysate
  Lane2: 231 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/1500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 30 seconds
  
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31244(MYL9 Antibody) at dilution 1/80, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting