• Product NameHCRTR2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total HCRTR2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide peptide corresponding to a region derived from 405-419 amino acids of human hypocretin (orexin) receptor 2
• Target NameHCRTR2
• Alternative Names    hypocretin (orexin) receptor 2, OX2R
• Accession No. Swiss-Prot:O43614
  Gene ID:3062;
• UniprotO43614
• Gene ID3062;
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 51kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.

• Gel: 10% SDS-PAGE
  Lane1: Mouse kidney tissue lysate
  Lane2: Mouse brain tissue lysate
  Lane3: Mouse heart tissue lysate
  Lysates: 40ug per lane
  Primary antibody: 1/1100 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 90 seconds
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting