• Product NamePECAM1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PECAM1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 725-735 amino acids of Human platelet/endothelial cell adhesion molecule 1
• Target NamePECAM1
• Alternative Names    Platelet/endothelial cell adhesion molecule 1, CD31, PECA1, GPIIA', PECAM-1, endoCAM, CD31/EndoCAM
• Accession No. Swiss-Prot:P16284
  Gene ID:5175;
• UniprotP16284
• Gene ID5175;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 83kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

• Gel: 8%SDS-PAGE
  Lysate: 60 µg Jurkat cell lysate
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 5 minutes
• The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31257 (PECAM1 Antibody) at dilution 1/30, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting