• Product NamePIBF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PIBF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide peptide corresponding to a region derived from 745-757 amino acids of human progesterone immunomodulatory binding factor 1
• Target NamePIBF1
• Alternative Names    progesterone immunomodulatory binding factor 1, PIBF, CEP90, C13orf24, RP11-505F3.1
• Accession No. Swiss-Prot:Q8WXW3
  Gene ID:10464;
• UniprotQ8WXW3
• Gene ID10464;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 90kd
  ELISA: 1:1000-1:2000
  Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Progesterone-induced-blocking factor 1 is a protein that in humans is encoded by the PIBF1 gene. It has been shown to localize to the centrosome and has also been named CEP90. Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.

• Gel: 10% SDS-PAGE
  Lane1: Hela cell lysate
  Lane2: 293T cell lysate
  Lysates: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 2 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31261 (PIBF1 Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting