• Product NameNIT2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total NIT2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 264-276 amino acids of Human itrilase family, member 2
• Target NameNIT2
• Alternative Names    Itrilase family, member 2
• Accession No. Swiss-Prot:Q9NQR4
  Gene ID:56954;
• UniprotQ9NQR4
• Gene ID56954;
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 31kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

• Gel: 10%SDS-PAGE
  Lane1: Mouse liver tissue lysate
  Lane2: Mouse kidney tissue lysate
  Lane3: RAW264.7 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/950 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 90 seconds seconds
  
• The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31280 (NIT2 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting