• Product NamePARP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PARP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 12-21 amino acids of human poly (ADP-ribose) polymerase 1
• Target NamePARP1
• Alternative Names    poly (ADP-ribose) polymerase 1, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
• Accession No. Swiss-Prot:P09874
  Gene ID:142;
• UniprotP09874
• Gene ID142;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 113kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

• Gel: 8%SDS-PAGE
  Lane1: Hela cell lysate
  Lane2: K562 cell lysate
  Lane3: Jurkat cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 second
  
• The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using 31288 (PARP1 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting