• Product NameHDAC4(Ab-632) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total HDAC4 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 630~634 (A-Q-S-S-P) derived from Human HDAC4.
• Target NameHDAC4
• Alternative Names    HD4; Histone deacetylase 4;
• Accession No. Swiss-Prot: P56524
  NCBI Protein: NP_006028.2
• UniprotP56524
• Gene ID9759;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 140kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Wang AH, et al. (2000) Mol Cell Biol. 20(18): 6904-6912.
Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97(14): 7835-7840.

• Western blot analysis of extracts from HepG2 cells using HDAC4(Ab-632) Antibody #21141.
• Immunofluorescence staining of methanol-fixed Hela cells using HDAC4(Ab-632) Antibody #21141.
• Western blotting analysis using HDAC4(Ab-632) Antibody #21141.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting