• Product NameBCL-2 (Phospho-Thr69) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB, IH, ELISA
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of BCL-2 only when phosphorylated at Threonine 69.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Threonine 69 (A-R-T(p)-S-P) derived from Human BCL-2.
• Target NameBCL-2
• ModificationPhospho
• Alternative Names    PPP1R50, BCL2
• Accession No. Swiss-Prot#: P10415;
  NCBI Gene#: 596;
  NCBI Protein#: NP_000624.2
• UniprotP10415
• Gene ID596;
• SDS-PAGE MW 26kd
• Concentration 1.0mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Store at -20˚C/1 year

• WB (1/500 - 1/1000), IH (1/100 - 1/200)

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

• Western blot analysis of BCL2 (pT69) expression in HEK293T (A), K562 (B), A549 (C), rat kidney (D), rat muscle (E) whole cell lysates.
• Immunohistochemical analysis of BCL2 (pT69) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• Direct ELISA antibody dose-response curve using Anti-BCL2 (pT69) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting