• Product NameBmi1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.10B1-D1
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of Bmi1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human Bmi1 protein fragments expressed in E.coli.
• Target NameBmi1
• Alternative Names    B lymphoma Mo MLV insertion region (mouse); B lymphoma Mo MLV insertion region 1 homolog;Bmi 1; BMI1; BMI1 polycomb ring finger oncogene; BMI1_HUMAN; Flvi 2/bmi 1; FLVI2/BMI1; MGC12685; Murine leukemia viral (bmi 1) oncogene homolog;Oncogene BMI 1; PCGF 4
• Accession No. UniProt: P35226

  Gene ID: 648

• UniprotP35226
• Gene ID100532731;648;
• SDS-PAGE MW 43kd
• Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

 Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

• Western blot detection of Bmi1 antibody in Hela,A549 and 293T cell lysates using Bmi1 antibody (1:1000 diluted).Predicted band size:43KDa.Observed band size:43KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting