• Product Namep53 (C-terminus) Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.6C4-C12-H10
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of p53 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human p53 protein fragments expressed in E.coli.
• Target Namep53
• Alternative Names    P53; LFS1; TRP53; TP53
• Accession No. Uniprot: P04637

  Gene ID: 7157

• UniprotP04637
• Gene ID7157;
• SDS-PAGE MW 53kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:1000

 Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

• Western blot detection of TP53 in SW480,T47D and MDA-MB-468 cell lysates using P53 antibody (1:1000 diluted).Predicted band size: 53KDa.Observed band size: 53KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting