• Product NameHsp27 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7E5-1F9-D9
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu Mk
• SpecificityThis antibody detects endogenous levels of HSPB1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human HSPB1 protein fragments expressed in E.coli.
• Target NameHsp27
• Alternative Names    28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27; HSPB1; HspB1; Hsp B1;
• Accession No. Uniprot: P04792

  Gene ID: 3315

• UniprotP04792
• Gene ID3315;
• SDS-PAGE MW 27kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:300

 Involved in stress resistance and actin organization.

• Immunocytochemistry stain of Hela using HSPB1 antibody (1:300).
• Western blot detection of HSPB1 in Hela,HsHela,A431，COS7，LNCAP&MCF7 cell lysates and using HSPB1 antibody (1:1000 diluted). Predicted band size: 23KDa Observed band size:27KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting