• Product NameSirT1 (Phospho-Ser47) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of SirT1 only when phosphorylated at serine 47.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 47(E-R-S(p)-P-G) derived from Human SirT1.
• Target NameSirT1
• ModificationPhospho
• Alternative Names    hSIR2; hSIRT1; NAD-dependent deacetylase sirtuin 1; SIR1; SIR2L1
• Accession No. Swiss-Prot#: Q96EB6;
  NCBI Gene#: 23411;
  NCBI Protein#: NP_001135970.1
• UniprotQ96EB6
• Gene ID23411;
• SDS-PAGE MW 110kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively.

Gao Z, et al. (2011) J Biol Chem 286, 22227-34. Zschoernig B, Mahlknecht U (2009)Biochem Biophys Res Commun 381, 372-7. Nasrin N, et al. (2009)PLoS One 4, e8414.

• Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) antibody #11926.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting